PKM2-bound RNAs in HeLa cells were obtained. To study the potential function of PKM2 in OS, Their apoptosis has become a new direction in the treatment of Proliferation, migration, and invasion of cancer cells and promote Regulation of PKM2 by multiple pathways to inhibit the Of PKM2 as an RBP in OS, have not been studied. Pyruvate kinase pre-mRNA and favors splicing of the oncogenic PKM2Īssociation between PKM2 and OS has been previously reported Tract binding protein 1 (PTBP1), which in turn is recruited to the (DR) phenotype relies on the upregulation of the polypyrimidine Through direct binding to specific sequenceĮlement(s), RNA binding proteins (RBPs) play a pivotal role in co-Īnd post-transcriptional RNA regulatory events ( 16). Signaling cascade plays important roles in the regulation of OS OS stem cells to cisplatin by inhibiting the expression of PKM2 The overexpression of PKM2 predictsĪ poor prognosis for patients with OS, and PKM2 may serve as aĪnother study confirmed that metformin increased the sensitivity of Participate in gene transcription regulation ( 10- 12). Regulating metabolism, PKM2 can also enter the nucleus and That PKM2 is overexpressed in cancer tissues and, in addition to
Pyruvate kinase M2 (PKM2) is a keyĮnzyme involved in glycolysis ( 9). Regardless of whether they are in an anoxic environment, which isĪlso known as aerobic glycolysis or the Warburg effect ( 8). AFAP1-AS1 could serve as a promisingĬancer cells use glycolysis for energy supply Indicated a novel molecular mechanism underlying the tumorigenesisĪnd progression of OS.
RhoC/ROCK1/p38MAPK/Twist1 signaling pathway, these findings Osteosarcoma and plays an oncogenic role in OS through the With the advancement of research, a variety of lncRNAs can be usedĪs potential prognostic indicators, biomarkers, and therapeutic Previous studies by our group have shown that lncRNAs, asĬompetitive endogenous RNAs or 'molecular baits' regulate theĮxpression of miRNAs and are involved in the occurrence,ĭevelopment, and metastasis of OS ( 6, 7). Targeting microRNAs (miRNAs or miRs), signaling pathways, Remodeling, and post-transcriptional regulation ( 4). Processes and phenotypes, including gene expression, chromatin Role in OS and can significantly regulate pathophysiological Long non-coding RNAs (lncRNAs) play an important Than one year, and the 5-year survival rate is often less than 20%Įlucidate the molecular mechanisms underlying the occurrence andĭevelopment of OS and to search for new molecular markers and Patients with recurrence or pulmonary metastasis is generally less Literature, the 5-year survival rate for patients with OS isīetween 37.5 and 77.6% however, the average survival time of Immunotherapy, but the prognosis of patients has not significantly Surgical resection, neoadjuvant chemotherapy, radiotherapy, and Currently, the main treatment methods include Malignant bone tumor in children and adolescents, and it isĬharacterized by malignant spindle stromal cells ( 1). Osteosarcoma (OS) is the most common primary This further extends the biological functions of PKM2 in tumorigenesis and makes it a novel potential therapeutic for OS. PKM2 appears to be a key regulator in OS by binding to lncCCAT1. These factors promoted the Warburg effect, lipogenesis, and OS cell growth. Validation of the PKM2‑lncRNA interaction in the human OS cell line revealed that lncRNA colon cancer associated transcript‑1 (lncCCAT1) interacted with PKM2, which upregulated the phosphorylation of sterol regulatory element‑binding protein 2 (SREBP2). Peak calling analysis revealed that PKM2 binds to long noncoding RNAs (lncRNAs), which are associated with cancer pathogenesis and development.
To study this, PKM2‑bound RNAs in HeLa cells, a type of cancer cells widely used in the study of molecular function and mechanism, were obtained. The association of PKM2 with osteosarcoma (OS) has been reported but its role in OS has yet to be elucidated. Pyruvate kinase M2 (PKM2) plays an important role in the consumption of glucose and the production of lactic acid, the striking feature of cancer metabolism.
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